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SMYD3 antibody

GTX121945
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetSMYD3
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMYD3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.23 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64754
  • Target name
    SMYD3
  • Target description
    SET and MYND domain containing 3
  • Target synonyms
    bA74P14.1; histone-lysine N-methyltransferase SMYD3; KMT3E; SET and MYND domain-containing protein 3; zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1; ZMYND1; ZNFN3A1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H7B4
  • Protein Name
    Histone-lysine N-methyltransferase SMYD3
  • Scientific Description
    SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A Positive Feedback Loop of lncRNA HOXD-AS2 and SMYD3 Facilitates Hepatocellular Carcinoma Progression via the MEK/ERK Pathway.
    Read more
  • The Lysine Methylase SMYD3 Modulates Mesendodermal Commitment during Development. Fittipaldi R et al., 2021 May 18, Cells
    Read more
  • Smyd3-PARP16 axis accelerates unfolded protein response and vascular aging. Yang D et al., 2020 Nov 3, Aging (Albany NY)
    Read more
  • SMYD3 promotes the epithelial-mesenchymal transition in breast cancer. Fenizia C et al., 2019 Feb 20, Nucleic Acids Res
    Read more
  • SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression. Chen YJ et al., 2017 Jun 19, Sci Rep
    Read more
  • The methyltransferase SMYD3 mediates the recruitment of transcriptional cofactors at the myostatin and c-Met genes and regulates skeletal muscle atrophy. Proserpio V et al., 2013 Jun 1, Genes Dev
    Read more

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Figure 1. Western blot analysis of SMYD3 using anti-SMYD3 antibody (PB9893). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMYD3 antigen affinity purified polyclonal antibody (Catalog # PB9893) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMYD3 at approximately 55 kDa. The expected band size for SMYD3 is at 49 kDa.
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