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SMYD3 Antibody (YA2003, Rabbit)

HY-P82258
MedChem Express
TargetSMYD3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SMYD3 Antibody (YA2003, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID64754
  • Target name
    SMYD3
  • Target description
    SET and MYND domain containing 3
  • Target synonyms
    KMT3E, ZMYND1, ZNFN3A1, bA74P14.1, histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, zinc finger MYND domain-containing protein 1, zinc finger protein, subfamily 3A (MYND domain containing), 1, zinc finger, MYND domain containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SMYD3 Antibody (YA2003) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2003), targeting SMYD3, with a predicted molecular weight of 49 kDa (observed band size: 49 kDa). SMYD3 Antibody (YA2003) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMYD3 using anti-SMYD3 antibody (PB9893). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMYD3 antigen affinity purified polyclonal antibody (Catalog # PB9893) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMYD3 at approximately 55 kDa. The expected band size for SMYD3 is at 49 kDa.
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