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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA021873ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA021873ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA021873ESR1HU at dilution of 1:100

SNAP25 Antibody

CSB-PA021873ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSNAP25
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNAP25 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6616
  • Target name
    SNAP25
  • Target description
    synaptosome associated protein 25
  • Target synonyms
    bA416N4.2; CMS18; dJ1068F16.2; resistance to inhibitors of cholinesterase 4 homolog; RIC4; RIC-4; SEC9; SNAP; SNAP-25; SUP; synaptosomal-associated protein 25; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60880
  • Protein Name
    Synaptosomal-associated protein 25
  • Scientific Description
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNAP25 using anti-SNAP25 antibody (A01625-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP25 antigen affinity purified polyclonal antibody (Catalog # A01625-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP25 at approximately 24 kDa. The expected band size for SNAP25 is at 24 kDa.
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