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Western Blot analysis of Jurkat cells using TudorSN Polyclonal Antibody
Western Blot analysis of Jurkat cells using TudorSN Polyclonal Antibody
Western Blot analysis of Jurkat cells using TudorSN Polyclonal Antibody

SND1 Antibody

CSB-PA004724
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSND1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SND1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27044
  • Target name
    SND1
  • Target description
    staphylococcal nuclease and tudor domain containing 1
  • Target synonyms
    TDRD11, TSN, Tudor-SN, p100, staphylococcal nuclease domain-containing protein 1, EBNA2 coactivator p100, testis tissue sperm-binding protein Li 82P, tudor domain-containing protein 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7KZF4
  • Protein Name
    Staphylococcal nuclease domain-containing protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SND1 using anti-SND1 antibody (A02602-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human HUVEC whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SND1 antigen affinity purified polyclonal antibody (Catalog # A02602-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SND1 at approximately 110KD. The expected band size for SND1 is at 110KD.
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