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ProductsBack/SND1 Antibody

SND1 Antibody

LS-C748465
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSND1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SND1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 101kDa, while the observed MW by Western blot was 102kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 101kDa, while the observed MW by Western blot was 102kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27044
  • Target name
    SND1
  • Target description
    staphylococcal nuclease and tudor domain containing 1
  • Target synonyms
    TDRD11, TSN, Tudor-SN, p100, staphylococcal nuclease domain-containing protein 1, EBNA2 coactivator p100, testis tissue sperm-binding protein Li 82P, tudor domain-containing protein 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SND1 using anti-SND1 antibody (A02602-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human HUVEC whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SND1 antigen affinity purified polyclonal antibody (Catalog # A02602-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SND1 at approximately 110KD. The expected band size for SND1 is at 110KD.
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