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Western Blot analysis of HeLa cells using SNRPN Polyclonal Antibody.
Western Blot analysis of HeLa cells using SNRPN Polyclonal Antibody.
Western Blot analysis of HeLa cells using SNRPN Polyclonal Antibody.

SNRPN Antibody

CSB-PA011056
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSNRPN
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNRPN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6638
  • Target name
    SNRPN
  • Target description
    small nuclear ribonucleoprotein polypeptide N
  • Target synonyms
    HCERN3; PWCR; RT-LI; sm protein D; SM protein N; small nuclear ribonucleoprotein-associated protein N; SM-D; SMN; sm-N; SNRNP-N; SNURF-SNRPN; tissue-specific splicing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63162
  • Protein Name
    Small nuclear ribonucleoprotein-associated protein N
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNRPN using anti-SNRPN antibody (PB9441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPN antigen affinity purified polyclonal antibody (Catalog # PB9441) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 25 kDa. The expected band size for SNRPN is at 25 kDa.
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