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SNRPN Monoclonal Antibody

BSM-60533M
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNRPN
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Overview

  • Supplier
    Bioss
  • Product Name
    SNRPN Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A3B1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6638
  • Target name
    SNRPN
  • Target description
    small nuclear ribonucleoprotein polypeptide N
  • Target synonyms
    HCERN3, PWCR, RT-LI, SM-D, SMN, SNRNP-N, SNURF-SNRPN, sm-N, small nuclear ribonucleoprotein-associated protein N, SM protein N, sm protein D, tissue-specific splicing protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP63162
  • Protein Name
    Small nuclear ribonucleoprotein-associated protein N
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNRPN using anti-SNRPN antibody (PB9441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPN antigen affinity purified polyclonal antibody (Catalog # PB9441) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 25 kDa. The expected band size for SNRPN is at 25 kDa.
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