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IHC-P analysis of human spleen tissue using GTX31617 SNRPN antibody. Working concentration : 5 microg/ml
IHC-P analysis of human spleen tissue using GTX31617 SNRPN antibody. Working concentration : 5 microg/ml
IHC-P analysis of human spleen tissue using GTX31617 SNRPN antibody. Working concentration : 5 microg/ml

SNRPN antibody

GTX31617
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSNRPN
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Overview

  • Supplier
    GeneTex
  • Product Name
    SNRPN antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6638
  • Target name
    SNRPN
  • Target description
    small nuclear ribonucleoprotein polypeptide N
  • Target synonyms
    HCERN3; PWCR; RT-LI; sm protein D; SM protein N; small nuclear ribonucleoprotein-associated protein N; SM-D; SMN; sm-N; SNRNP-N; SNURF-SNRPN; tissue-specific splicing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63162
  • Protein Name
    Small nuclear ribonucleoprotein-associated protein N
  • Scientific Description
    The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5 untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5 UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNRPN using anti-SNRPN antibody (PB9441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPN antigen affinity purified polyclonal antibody (Catalog # PB9441) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 25 kDa. The expected band size for SNRPN is at 25 kDa.
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