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Goat anti-SNX1 Antibody

ORB20425
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetSNX1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-SNX1 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 75kDa band observed in Human lysates of cell line HeLa (calculated MW of 59.1kDa according to NP_003090.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 microg/ml.
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 3-5 μg/ml ELISA, ICC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6642
  • Target name
    SNX1
  • Target description
    sorting nexin 1
  • Target synonyms
    HsT17379, VPS5, sorting nexin-1, sorting nexin 1A
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to SNX1
  • Reactivity
    Bovine, Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SNX1 using anti-SNX1 antibody (A02692-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX1 antigen affinity purified polyclonal antibody (Catalog # A02692-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX1 at approximately 70-75 kDa. The expected band size for SNX1 is at 59 kDa.
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