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ProductsBack/SNX25 Antibody

SNX25 Antibody

LS-C749326
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSNX25
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SNX25 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 64kDa/97kDa, while the observed MW by Western blot was 100kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 64kDa/97kDa, while the observed MW by Western blot was 100kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID83891
  • Target name
    SNX25
  • Target description
    sorting nexin 25
  • Target synonyms
    LRP2BP-AS1, MSTP043, SBBI31, sorting nexin-25, LRP2BP antisense RNA 1, RP11-714G18.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA938282(SNX25 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of SNX25 using anti-SNX25 antibody (A13669). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX25 antigen affinity purified polyclonal antibody (Catalog # A13669) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX25 at approximately 100 kDa. The expected band size for SNX25 is at 98 kDa.
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