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ProductsBack/SNX25 antibody [N2C1], Internal
SNX25 antibody detects SNX25 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE SNX25 antibody (GTX118491) dilution: 1:1000
SNX25 antibody detects SNX25 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE SNX25 antibody (GTX118491) dilution: 1:1000
SNX25 antibody detects SNX25 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE SNX25 antibody (GTX118491) dilution: 1:1000

SNX25 antibody [N2C1], Internal

GTX118491
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSNX25
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Overview

  • Supplier
    GeneTex
  • Product Name
    SNX25 antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.92 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID83891
  • Target name
    SNX25
  • Target description
    sorting nexin 25
  • Target synonyms
    LRP2BP-AS1, MSTP043, SBBI31, sorting nexin-25, LRP2BP antisense RNA 1, RP11-714G18.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3E2
  • Protein Name
    Sorting nexin-25
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNX25 using anti-SNX25 antibody (A13669). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX25 antigen affinity purified polyclonal antibody (Catalog # A13669) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX25 at approximately 100 kDa. The expected band size for SNX25 is at 98 kDa.
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