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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: SNX9 antibody at 6.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: SNX9 antibody at 6.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: SNX9 antibody at 6.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa

SNX9 Antibody

CSB-PA896917LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSNX9
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNX9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51429
  • Target name
    SNX9
  • Target description
    sorting nexin 9
  • Target synonyms
    SDP1; SH3 and PX domain-containing protein 1; SH3 and PX domain-containing protein 3A; SH3PX1; SH3PXD3A; sorting nexin-9; Wiskott-Aldrich syndrome protein (WASP) interactor protein; WISP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5X1
  • Protein Name
    Sorting nexin-9
  • Scientific Description
    Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SH3PX1/SNX9 using anti-SH3PX1/SNX9 antibody (A03796-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3PX1/SNX9 antigen affinity purified polyclonal antibody (Catalog # A03796-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3PX1/SNX9 at approximately 78 kDa. The expected band size for SH3PX1/SNX9 is at 67 kDa.
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