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SOCS4 Rabbit Polyclonal Antibody

ES10319
ELK Biotechnology
Product group Antibodies

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Overview

  • Supplier
    ELK Biotechnology
  • Product Name
    SOCS4 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOCS4 using anti-SOCS4 antibody (PB9506). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: SW620 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: SKOV Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS4 antigen affinity purified polyclonal antibody (Catalog # PB9506) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS4 at approximately 51 kDa. The expected band size for SOCS4 is at 51 kDa.
Product group Antibodies
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Everest Biotech
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GeneTex
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ReactivityHuman
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Product group Antibodies
Antibodies.com
Anti-SOCS4 AntibodyA12195
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ReactivityHuman, Mouse
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Western blot All lanes: SOCS4 antibody at 4.39 ug/ml Lane 1: Mouse kidney tissue Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Product group Antibodies
Cusabio
SOCS4 AntibodyCSB-PA022393ESR1HU
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ReactivityHuman, Mouse
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Immunofluorescent staining of human cell line A549 shows localization to nucleus & cytosol.
Product group Antibodies
Atlas Antibodies
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Product group Antibodies
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