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ProductsBack/SOD1 / Cu-Zn SOD Antibody (clone 10, Preservative Free)

SOD1 / Cu-Zn SOD Antibody (clone 10, Preservative Free)

LS-C820229
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetSOD1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SOD1 / Cu-Zn SOD Antibody (clone 10, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap): This antibody will detect Human SOD1 in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Applications Supplier
    ELISA ELISA(Cap): This antibody will detect Human SOD1 in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6647
  • Target name
    SOD1
  • Target description
    superoxide dismutase 1
  • Target synonyms
    ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer, superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SOD, soluble, epididymis secretory protein Li 44, indophenoloxidase A, superoxide dismutase 1, soluble, superoxide dismutase, cystolic
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOD1 using anti-SOD1 antibody (PB9402). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PB9402) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.
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