ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1microg/ml (12 hours at 4oC)
![IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibodys epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in Appendix. Antibody amount : MS27 (GTX57212): 2microg, MS785 (GTX57211): 5microg, or MS27/MS785 cocktail: 1microg IP lysis buffer: 1% Triton X100/TBS buffer.](https://www.genetex.com/upload/website/prouct_img/normal/GTX57212/GTX57212_IP_1-1_w_23061123_188.webp "IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibodys epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in Appendix. Antibody amount : MS27 (GTX57212): 2microg, MS785 (GTX57211): 5microg, or MS27/MS785 cocktail: 1microg IP lysis buffer: 1% Triton X100/TBS buffer.")
![IP analysis of HEK293 cells cultured in the presence and absence of 10microM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation. Antibody amount : 1-5 microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX57212/GTX57212_IP_2_w_23061123_930.webp "IP analysis of HEK293 cells cultured in the presence and absence of 10microM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation. Antibody amount : 1-5 microg")
ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1microg/ml (12 hours at 4oC)
SOD1 (mutant) antibody [MS27]
GTX57212
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSOD1
Overview
- SupplierGeneTex
- Product NameSOD1 (mutant) antibody [MS27]
- Delivery Days Customer9
- Antibody SpecificityClone MS785 and MS27, which specifically bind to conformationally altered SOD1 mutants, not detecting wild type SOD1 homo-dimer. Both clones are succeeded in specifically detecting over 100 SOD1 mutants (please see to Appendix for detail description).
- Application Supplier NoteICC/IF: 1 microg/ml (1/500 dilution) -5 microg/ml (1/100 dilution). IP: 1-5microg/sample. IHC: 1 microg/ml (1/500 dilution) -5 microg/ml (1/100 dilution). *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDMS27
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID6647
- Target nameSOD1
- Target descriptionsuperoxide dismutase 1
- Target synonymsALS; ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; homodimer; hSod1; indophenoloxidase A; IPOA; SOD; SOD, soluble; STAHP; superoxide dismutase [Cu-Zn]; superoxide dismutase 1, soluble; superoxide dismutase, cystolic
- HostRat
- IsotypeIgG2a
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants. Fujisawa T et al., 2015 Oct, Neurobiol DisRead more