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Western Blot analysis of NIH-3T3 cells using SOD-2 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using SOD-2 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using SOD-2 Polyclonal Antibody

SOD2 Antibody

CSB-PA006154
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SOD2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6648
  • Target name
    SOD2
  • Target description
    superoxide dismutase 2
  • Target synonyms
    GC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, lncRNA-GC1, superoxide dismutase [Mn], mitochondrial, Mn superoxide dismutase, epididymis secretory sperm binding protein, gastric cancer-associated lncRNA 1, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04179
  • Protein Name
    Superoxide dismutase [Mn], mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
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Figure 1. Western blot analysis of SOD2 using anti-SOD2 antibody (PB9442). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (Catalog # PB9442) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 24 kDa. The expected band size for SOD2 is at 25 kDa.
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