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Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD

SOD2 Polyclonal Antibody

BS-1080R
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSOD2
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Overview

  • Supplier
    Bioss
  • Product Name
    SOD2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6648
  • Target name
    SOD2
  • Target description
    superoxide dismutase 2
  • Target synonyms
    GC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, lncRNA-GC1, superoxide dismutase [Mn], mitochondrial, Mn superoxide dismutase, epididymis secretory sperm binding protein, gastric cancer-associated lncRNA 1, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04179
  • Protein Name
    Superoxide dismutase [Mn], mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Faria AVS, Clerici SP, de Souza Oliveira PF, et al. LMWPTP modulates the antioxidant response and autophagy process in human chronic myeloid leukemia cells. Mol Cell Biochem. 2020,466(1-2):83-89. doi: 10.1007/s11010-020-03690-1
    Read this paper
  • Gao J, Li T, Lu Z, et al. Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep. Animals (Basel). 2019,9(6). doi: 10.3390/ani9060309
    Read this paper
  • Zhang W, Zhao Y, Zhang P, et al. Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Chemosphere. 2018,194:147-157. doi: 10.1016/j.chemosphere.2017.11.164
    Read this paper
  • Yun Y, Gao R, Yue H, et al. Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT). Environ Sci Technol. 2017,51(19):11401-11411. doi: 10.1021/acs.est.7b02857
    Read this paper
  • Zhao Y, Zhang WD, Liu XQ, et al. Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways. Sci Rep. 2016,6:37884. doi: 10.1038/srep37884
    Read this paper
  • Wang Y, Zhao Y, Yu S, et al. Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Toxicol Appl Pharmacol. 2016,313:149-158. doi: 10.1016/j.taap.2016.10.025
    Read this paper

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Figure 1. Western blot analysis of SOD2 using anti-SOD2 antibody (PB9442). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (Catalog # PB9442) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 24 kDa. The expected band size for SOD2 is at 25 kDa.
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