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ProductsBack/SOD2 Polyclonal Antibody
Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Mouase heart lysates probed with Anti-SOD2 Polyclonal Antibody(bs-1080R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD

SOD2 Polyclonal Antibody

BS-1080R
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSOD2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SOD2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6648
  • Target name
    SOD2
  • Target description
    superoxide dismutase 2
  • Target synonyms
    epididymis secretory sperm binding protein; gastric cancer-associated lncRNA 1; GClnc1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; MNSOD; Mn-SOD; MVCD6; superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04179
  • Protein Name
    Superoxide dismutase [Mn], mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • LMWPTP modulates the antioxidant response and autophagy process in human chronic myeloid leukemia cells. Faria AVS et al., 2020 Mar, Mol Cell Biochem
    Read more
  • Proteomic Analyses of Mammary Glands Provide Insight into the Immunity and Metabolism Pathways Associated with Clinical Mastitis in Meat Sheep. Gao J et al., 2019 May 31, Animals (Basel)
    Read more
  • Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Zhang W et al., 2018 Mar, Chemosphere
    Read more
  • Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT). Yun Y et al., 2017 Oct 3, Environ Sci Technol
    Read more
  • Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways. Zhao Y et al., 2016 Nov 24, Sci Rep
    Read more
  • Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Wang Y et al., 2016 Dec 15, Toxicol Appl Pharmacol
    Read more

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Figure 1. Western blot analysis of SOD2 using anti-SOD2 antibody (PB9442). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (Catalog # PB9442) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 24 kDa. The expected band size for SOD2 is at 25 kDa.
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