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ProductsBack/Sodium hydrogen exchanger 1 Polyclonal Antibody
L1 rat brain, L2 rat liver lysates probed (bs-0505R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 91kDa.
L1 rat brain, L2 rat liver lysates probed (bs-0505R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 91kDa.
L1 rat brain, L2 rat liver lysates probed (bs-0505R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 91kDa.

Sodium hydrogen exchanger 1 Polyclonal Antibody

BS-0505R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC9A1
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Overview

  • Supplier
    Bioss
  • Product Name
    Sodium / Hydrogen Exchanger 1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6548
  • Target name
    SLC9A1
  • Target description
    solute carrier family 9 member A1
  • Target synonyms
    APNH, LIKNS, NHE-1, NHE1, PPP1R143, sodium/hydrogen exchanger 1, Na(+)/H(+) exchanger 1, Na-Li countertransporter, protein phosphatase 1, regulatory subunit 143, solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive), solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19634
  • Protein Name
    Sodium/hydrogen exchanger 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of SLC9A1 using anti-SLC9A1 antibody (PB9151). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Sodium protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A1 antigen affinity purified polyclonal antibody (Catalog # PB9151) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A1 at approximately 37 kDa. The expected band size for SLC9A1 is at 37 kDa.
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