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ProductsBack/Sorbitol Dehydrogenase (SORD) Mouse Monoclonal Antibody [Clone ID: OTI12A7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SORD (RC200415, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SORD. Positive lysates LY401082 (100ug) and LC401082 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SORD (RC200415, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SORD. Positive lysates LY401082 (100ug) and LC401082 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SORD (RC200415, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SORD. Positive lysates LY401082 (100ug) and LC401082 (20ug) can be purchased separately from OriGene.

Sorbitol Dehydrogenase (SORD) Mouse Monoclonal Antibody [Clone ID: OTI12A7]

CF500677
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSORD
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Sorbitol Dehydrogenase (SORD) Mouse Monoclonal Antibody [Clone ID: OTI12A7]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI12A7
  • Gene ID6652
  • Target name
    SORD
  • Target description
    sorbitol dehydrogenase
  • Target synonyms
    HEL-S-95n, HMNR8, RDH, SDH, SORD1, SORDD, XDH, sorbitol dehydrogenase, (R,R)-butanediol dehydrogenase, L-iditol 2-dehydrogenase, epididymis secretory sperm binding protein Li 95n, polyol dehydrogenase, ribitol dehydrogenase, xylitol dehydrogenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00796
  • Protein Name
    Sorbitol dehydrogenase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SORD mouse monoclonal antibody, clone OTI12A7 (formerly 12A7)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (A07851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD antigen affinity purified polyclonal antibody (Catalog # A07851-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/ SORD at approximately 43KD. The expected band size for Sorbitol Dehydrogenase/ SORD is at 38KD.
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