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Western Blot analysis of COS7 cells using Sox-8/9/17/18 Polyclonal Antibody
Western Blot analysis of COS7 cells using Sox-8/9/17/18 Polyclonal Antibody
Western Blot analysis of COS7 cells using Sox-8/9/17/18 Polyclonal Antibody

SOX8/SOX9/SOX17/SOX18 Antibody

CSB-PA004133
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSOX9
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Overview

  • Supplier
    Cusabio
  • Product Name
    SOX8/SOX9/SOX17/SOX18 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6662
  • Target name
    SOX9
  • Target description
    SRY-box transcription factor 9
  • Target synonyms
    CMD1; CMPD1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35713
  • Protein Name
    Transcription factor SOX-18
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX9 using anti-SOX9 antibody (A00177-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates Lane 2: human PC-3 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified polyclonal antibody (Catalog # A00177-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70KD. The expected band size for SOX9 is at 56KD.
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