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SW480 and Ht29 lysates probed with SOX9 Polyclonal Antibody, unconjugated (bs-4177R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
SW480 and Ht29 lysates probed with SOX9 Polyclonal Antibody, unconjugated (bs-4177R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
SW480 and Ht29 lysates probed with SOX9 Polyclonal Antibody, unconjugated (bs-4177R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

SOX9 Polyclonal Antibody

BS-4177R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSOX9
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SOX9 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6662
  • Target name
    SOX9
  • Target description
    SRY-box transcription factor 9
  • Target synonyms
    CMD1; CMPD1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48436
  • Protein Name
    Transcription factor SOX-9
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Comparative Evaluation of the Book-Type Acellular Bone Scaffold and Fibrocartilage Scaffold for Bone-Tendon Healing. Lu H et al., 2019 Apr 12, J Orthop Res
    Read more
  • Intra-articular injection of N-acetylglucosamine and hyaluronic acid combined with PLGA scaffolds for osteochondral repair in rabbits. Wang HC et al., 2018, PLoS One
    Read more
  • Reelin regulates male mouse reproductive capacity via the sertoli cells. Zhuang M et al., 2018 Oct 18, J Cell Biochem
    Read more
  • Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats. Chen X et al., 2018 Dec 1, J Biomed Nanotechnol
    Read more
  • Evidence of BrdU-positive retinal neurons after application of an Alpha7 nicotinic acetylcholine receptor agonist. Webster MK et al., 2017 Mar 27, Neuroscience
    Read more
  • Platelet-rich plasma protects rat chondrocytes from interleukin-1beta-induced apoptosis. Yang J et al., 2016 Nov, Mol Med Rep
    Read more
  • Loss of ZFP36 expression in colorectal cancer correlates to wnt/ beta-catenin activity and enhances epithelial-to-mesenchymal transition through upregulation of ZEB1, SOX9 and MACC1. Montorsi L et al., 2016 Sep 13, Oncotarget
    Read more
  • Subchondral bone changes and chondrogenic capacity of progenitor cells from subchondral bone in the collagenase-induced temporomandibular joints osteoarthritis rabbit model. Wu G et al., 2015, Int J Clin Exp Pathol
    Read more
  • The repair of full-thickness articular cartilage defect using intra-articular administration of N-acetyl-D-glucosamine in the rabbit knee: randomized controlled trial. Chang NJ et al., 2015 Nov 18, Biomed Eng Online
    Read more
  • MicroRNA-140 Provides Robustness to the Regulation of Hypertrophic Chondrocyte Differentiation by the PTHrP-HDAC4 Pathway. Papaioannou G et al., 2015 Jun, J Bone Miner Res
    Read more

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Figure 1. Western blot analysis of SOX9 using anti-SOX9 antibody (A00177-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates Lane 2: human PC-3 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified polyclonal antibody (Catalog # A00177-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70KD. The expected band size for SOX9 is at 56KD.
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