More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SPARC Antibody
Western Blot analysis of HeLa HepG2 cells using SPARC Polyclonal Antibody
Western Blot analysis of HeLa HepG2 cells using SPARC Polyclonal Antibody
Western Blot analysis of HeLa HepG2 cells using SPARC Polyclonal Antibody

SPARC Antibody

CSB-PA006034
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSPARC
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    SPARC Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6678
  • Target name
    SPARC
  • Target description
    secreted protein acidic and cysteine rich
  • Target synonyms
    BM-40, OI17, ON, ONT, SPARC, basement-membrane protein 40, secreted protein, acidic, cysteine-rich (osteonectin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09486
  • Protein Name
    SPARC
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
Osteonectin (ON) Polyclonal AntibodyCAU27131
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetSPARC
  • SizePrice
Product group Antibodies
References
GeneTex
SPARC antibodyGTX133747
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPARC
  • SizePrice
Product group Antibodies
MedChem Express
SPARC Antibody (YA1202, Rabbit)HY-P81457
TargetSPARC
  • SizePrice
Anti-SPARC Antibody
Product group Antibodies
Antibodies.com
Anti-SPARC AntibodyA97211
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SPARC Antibody144-60680
ApplicationsWestern Blot
TargetSPARC
  • SizePrice
Product group Antibodies
Biosynth
SPARC antibody70R-14122
  • SizePrice
Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A375 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
Product group Antibodies
Boster Bio
Anti-SPARC Antibody Picoband(r)A00862-1-CARRIER-FREE
ApplicationsWestern Blot
TargetSPARC
  • SizePrice
Product group Antibodies
Assay Genie
SPARC AntibodyCAB14494
TargetSPARC
  • SizePrice
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Product group Antibodies
Bioss
SPARC Polyclonal AntibodyBS-1133R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSPARC
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
Osteonectin / SPARC Antibody (aa18-303)LS-C401048
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetSPARC
  • SizePrice