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ProductsBack/SPARC Rabbit Polyclonal Antibody
WB Suggested Anti-SPARC Antibody Titration: 1.0 ug/ml Positive Control: PANC1 Whole CellSPARC is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells
WB Suggested Anti-SPARC Antibody Titration: 1.0 ug/ml Positive Control: PANC1 Whole CellSPARC is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells
WB Suggested Anti-SPARC Antibody Titration: 1.0 ug/ml Positive Control: PANC1 Whole CellSPARC is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells

SPARC Rabbit Polyclonal Antibody

TA358367
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetSPARC
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SPARC Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6678
  • Target name
    SPARC
  • Target description
    secreted protein acidic and cysteine rich
  • Target synonyms
    basement-membrane protein 40; BM-40; OI17; ON; ONT; secreted protein, acidic, cysteine-rich (osteonectin); SPARC
  • Host
    Rabbit
  • Protein IDP09486
  • Protein Name
    SPARC
  • Scientific Description
    SPARC Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A375 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
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