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Western Blot analysis of A549 and lncap cell, Human seminoma tissue using SPATA20 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of A549 and lncap cell, Human seminoma tissue using SPATA20 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of A549 and lncap cell, Human seminoma tissue using SPATA20 Polyclonal Antibody at dilution of 1:1000

SPATA20 Polyclonal Antibody

E-AB-16072
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SPATA20 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.9mg/ml
  • Scientific Description
    The protein may play a role in fertility regulation. It were significantly upregulated in individual bile samples from Cholangiocarcinoma patients by Western blotting and Immunohistochemistry, relative to normal tissues.It acts as a potential serum diagnostic biomarker for Cholangiocarcinoma.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPATA20 using anti-SPATA20 antibody (A17005-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA20 antigen affinity purified polyclonal antibody (Catalog # A17005-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA20 at approximately 88 kDa. The expected band size for SPATA20 is at 88 kDa.
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Immunohistochemistry analysis in human testis and tonsil tissues using HPA027144 antibody. Corresponding SPATA20 RNA-seq data are presented for the same tissues.
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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA928166(SPATA20 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
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