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Immunohistochemistry of paraffin-embedded Human colon cancer tissue using SPATA7 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using SPATA7 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using SPATA7 Polyclonal Antibody at dilution 1:30

SPATA7 Polyclonal Antibody

E-AB-14378
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SPATA7 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.2mg/ml
  • Scientific Description
    This gene, originally isolated from testis, is also expressed in retina. Mutations in this gene are associated with Leber congenital amaurosis and juvenile retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPATA7 using anti-SPATA7 antibody (A08310-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA7 antigen affinity purified polyclonal antibody (Catalog # A08310-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA7 at approximately 68 kDa. The expected band size for SPATA7 is at 68 kDa.
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