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Western Blot analysis of 3T3 cells using CCDC99 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CCDC99 Polyclonal Antibody
Western Blot analysis of 3T3 cells using CCDC99 Polyclonal Antibody

SPDL1 Antibody

CSB-PA030153
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSPDL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPDL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54908
  • Target name
    SPDL1
  • Target description
    spindle apparatus coiled-coil protein 1
  • Target synonyms
    arsenite-related gene 1 protein; CCDC99; coiled-coil domain-containing protein 99; protein Spindly; rhabdomyosarcoma antigen MU-RMS-40.4A; rrhabdomyosarcoma antigen protein MU-RMS-40.4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EA4
  • Protein Name
    Protein Spindly
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Spindly/SPDL1 using anti-Spindly/SPDL1 antibody (A12722-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spindly/SPDL1 antigen affinity purified polyclonal antibody (Catalog # A12722-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spindly/SPDL1 at approximately 70 kDa. The expected band size for Spindly/SPDL1 is at 70 kDa.
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