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ProductsBack/SPFH2 / ERLIN2 Antibody

SPFH2 / ERLIN2 Antibody

LS-C409849
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetERLIN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPFH2 / ERLIN2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 16kDa/22kDa/37kDa, while the observed MW by Western blot was 38kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 16kDa/22kDa/37kDa, while the observed MW by Western blot was 38kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11160
  • Target name
    ERLIN2
  • Target description
    ER lipid raft associated 2
  • Target synonyms
    C8orf2, Erlin-2, NET32, SPFH2, SPG18, SPG18A, SPG18B, erlin-2, SPFH domain family, member 2, endoplasmic reticulum lipid raft-associated protein 2, epididymis secretory sperm binding protein, spastic paraplegia 18 (autosomal dominant), stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Erlin-2/ERLIN2 using anti-Erlin-2/ERLIN2 antibody (A07042-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat testis tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse kidney tissue lysates, Lane 10: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Erlin-2/ERLIN2 antigen affinity purified polyclonal antibody (Catalog # A07042-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Erlin-2/ERLIN2 at approximately 43 kDa. The expected band size for Erlin-2/ERLIN2 is at 43 kDa.
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