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ProductsBack/Sprouty 4 Antibody (YA1780, Rabbit)

Sprouty 4 Antibody (YA1780, Rabbit)

HY-P82035
MedChem Express
TargetSPRY4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Sprouty 4 Antibody (YA1780, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID81848
  • Target name
    SPRY4
  • Target description
    sprouty RTK signaling antagonist 4
  • Target synonyms
    HH17; protein sprouty homolog 4; sprouty homolog 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Sprouty 4 Antibody (YA1780) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1780), targeting Sprouty 4, with a predicted molecular weight of 33 kDa (observed band size: 33 kDa). Sprouty 4 Antibody (YA1780) can be used for WB, ICC/IF, IP, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sprouty 4/Spry-4/SPRY4 antigen affinity purified polyclonal antibody (Catalog # A04343-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sprouty 4/Spry-4/SPRY4 at approximately 35KD. The expected band size for Sprouty 4/Spry-4/SPRY4 is at 35KD.
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