More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SPRY4 Antibody
Immunofluorescence staining of PC-3 cells with CSB-PA866335LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cells with CSB-PA866335LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cells with CSB-PA866335LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SPRY4 Antibody

CSB-PA866335LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetSPRY4
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    SPRY4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81848
  • Target name
    SPRY4
  • Target description
    sprouty RTK signaling antagonist 4
  • Target synonyms
    HH17; protein sprouty homolog 4; sprouty homolog 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C004
  • Protein Name
    Protein sprouty homolog 4
  • Scientific Description
    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. Inhibits Ras-independent, but not Ras-dependent, activation of RAF1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Related products

Product group Antibodies
Abbexa
SPRY4-Y75 AntibodyABX033400
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Assay Genie
SPRY4 AntibodyCAB6263
TargetSPRY4
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Sprouty 4 / SPRY4 (aa306-322) Antibody119-11427
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPRY4
  • SizePrice
Product group Antibodies
Bioss Antibodies
Sprouty 4 Recombinant Antibody, AbBy Fluor-555 ConjugatedBSM-61880R-BF555
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetSPRY4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
Sprouty 4 / SPRY4 AntibodyLS-C831789
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetSPRY4
  • SizePrice
Immunofluorescent staining of human cell line PC-3 shows localization to cytosol & the Golgi apparatus.
Product group Antibodies
Atlas Antibodies
Anti-SPRY4 AntibodyHPA055471
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetSPRY4
  • SizePrice
Figure 1. Western blot analysis of Sprouty 4/Spry-4/SPRY4 using anti-Sprouty 4/Spry-4/SPRY4 antibody (A04343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sprouty 4/Spry-4/SPRY4 antigen affinity purified polyclonal antibody (Catalog # A04343-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sprouty 4/Spry-4/SPRY4 at approximately 35KD. The expected band size for Sprouty 4/Spry-4/SPRY4 is at 35KD.
Product group Antibodies
Boster Bio
Anti-Sprouty 4/Spry-4/SPRY4 Antibody Picoband(r)A04343-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetSPRY4
  • SizePrice
Product group Antibodies
GeneTex
SPRY4 antibodyGTX119230
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPRY4
  • SizePrice
Product group Antibodies
MedChem Express
Sprouty 4 Antibody (YA1780, Rabbit)HY-P82035
TargetSPRY4
  • SizePrice