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SPTBN2 Antibody

LS-C783405
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetSPTBN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPTBN2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo (2 - 4 µg/10E6 cells), WB (0.1 - 0.5 µg/ml) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6712
  • Target name
    SPTBN2
  • Target description
    spectrin beta, non-erythrocytic 2
  • Target synonyms
    beta-III spectrin; glutamate transporter EAAT4-associated protein 41; GTRAP41; SCA5; SCAR14; spectrin beta chain, brain 2; spectrin beta chain, non-erythrocytic 2; spectrin beta III sigma 2; spectrin, non-erythroid beta chain 2; spinocerebellar ataxia 5 protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTBN2 antigen affinity purified polyclonal antibody (Catalog # A05492) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPTBN2 at approximately 271 kDa. The expected band size for SPTBN2 is at 271 kDa.
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