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Western blot analysis of extracts of various cell lines using SQRDL Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SQRDL Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SQRDL Polyclonal Antibody at dilution of 1:3000.

SQRDL Polyclonal Antibody

E-AB-63946
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SQRDL Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene may function in mitochondria to catalyze the conversion of sulfide to persulfides, thereby decreasing toxic concencrations of sulfide. Alternative splicing results in multiple transcript variants that encode the same protein.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SQRDL/SQOR using anti-SQRDL/SQOR antibody (A31817). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQRDL/SQOR antigen affinity purified polyclonal antibody (Catalog # A31817) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQRDL/SQOR at approximately 50 kDa. The expected band size for SQRDL/SQOR is at 50 kDa.
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Western blot All lanes: SQRDL antibody at 4.67ug/ml Lane 1: A549 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa
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