HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:500).
SQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI1B2]
CF502199
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSQSTM1
Overview
- SupplierOriGene
- Product NameSQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI1B2]
- Delivery Days Customer14
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDOTI1B2
- Gene ID8878
- Target nameSQSTM1
- Target descriptionsequestosome 1
- Target synonymsA170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1, EBI3-associated protein of 60 kDa, EBI3-associated protein p60, autophagy receptor p62, oxidative stress induced like, phosphotyrosine independent ligand for the Lck SH2 domain p62, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62
- HostMouse
- IsotypeIgG1
- Protein IDQ13501
- Protein NameSequestosome-1
- Scientific DescriptionCarrier-free (BSA/glycerol-free) SQSTM1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203
MSDS
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![Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100685/GTX100685_44538_20220422_WB_M_treatment_Thapsigargin_w_23060100_424.webp)
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Anti-SQSTM1 AntibodyHPA068843
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