Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220812_WB_competitor_watermark_22081423_370.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220121_ICC_IF_w_23061202_882.webp "SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20211029_IHC-P_w_23061202_604.webp "SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220318_WB_M_w_23061202_930.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_44452_20220506_WB_M_treatment_Thapsigargin_w_23061202_406.webp "Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_45152_20230901_WB_25021320_420.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636328/GTX636328_45152_20230901_WB_treatment_Thapsigargin_25021320_797.webp "Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SQSTM1 / P62 antibody [HL1113]
GTX636328
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSQSTM1
Overview
- SupplierGeneTex
- Product NameSQSTM1 / P62 antibody [HL1113]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1113
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8878
- Target nameSQSTM1
- Target descriptionsequestosome 1
- Target synonymsA170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1, EBI3-associated protein of 60 kDa, EBI3-associated protein p60, autophagy receptor p62, oxidative stress induced like, phosphotyrosine independent ligand for the Lck SH2 domain p62, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62
- HostRabbit
- IsotypeIgG
- Protein IDQ13501
- Protein NameSequestosome-1
- Scientific DescriptionThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wang Y, Huang M, Xu W, et al. Calcitriol-enhanced autophagy in gingival epithelium attenuates periodontal inflammation in rats with type 2 diabetes mellitus. Front Endocrinol (Lausanne). 2022,13:1051374. doi: 10.3389/fendo.2022.1051374Read this paper
Datasheet
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