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SRD5A2 Polyclonal Antibody

BS-6700R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSRD5A2
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Overview

  • Supplier
    Bioss
  • Product Name
    SRD5A2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6716
  • Target name
    SRD5A2
  • Target description
    steroid 5 alpha-reductase 2
  • Target synonyms
    3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, S5AR 2, SR type 2, steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2), type II 5-alpha reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Song L, Cui Y, Xiao L, et al. DHT and E2 synthesis-related proteins and receptors expression in male yak skin during different hair follicle stages. Gen Comp Endocrinol. 2020,286:113245. doi: 10.1016/j.ygcen.2019.113245
    Read this paper
  • Xiao L, Song L, Hu J, et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019,234(9):15182-15193. doi: 10.1002/jcp.28159
    Read this paper

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Figure 1. Western blot analysis of SRD5A2 using anti-SRD5A2 antibody (M00704). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human NIH/3T3 whole cell lysates, Lane 6: mouse HT22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRD5A2 antigen affinity purified monoclonal antibody (Catalog # M00704) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRD5A2 at approximately 28 kDa. The expected band size for SRD5A2 is at 28 kDa.
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