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SRGAP3 Antibody, Biotin conjugated

CSB-PA022663LD01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetSRGAP3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRGAP3 Antibody, Biotin conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID9901
  • Target name
    SRGAP3
  • Target description
    SLIT-ROBO Rho GTPase activating protein 3
  • Target synonyms
    ARHGAP14, MEGAP, SRGAP2, WRP, SLIT-ROBO Rho GTPase-activating protein 3, SLIT-ROBO Rho GTPase activating protein 2, WAVE-associated Rac GTPase activating protein, mental disorder-associated GAP, rho GTPase-activating protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43295
  • Protein Name
    SLIT-ROBO Rho GTPase-activating protein 3
  • Scientific Description
    GTPase-activating protein for RAC1 and perhaps Cdc42, but not for RhoA small GTPase. May attenuate RAC1 signaling in neurons.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SRGAP3 using anti-SRGAP3 antibody (A06020-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRGAP3 antigen affinity purified polyclonal antibody (Catalog # A06020-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRGAP3 at approximately 140 kDa. The expected band size for SRGAP3 is at 125 kDa.
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Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-SRGAP3 antibody. Corresponding SRGAP3 RNA-seq data are presented for the same tissues.
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