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SRM Antibody Pair

CSB-EAP01115
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityCanine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
TargetSRM
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRM Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.2ug/ml and the detection antibody at a concentration of 0.125ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID6723
  • Target name
    SRM
  • Target description
    spermidine synthase
  • Target synonyms
    PAPT; putrescine aminopropyltransferase; SPDSY; spermidine synthase; spermidine synthase-1; SPS1; SRML1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19623
  • Protein Name
    Spermidine synthase
  • Reactivity
    Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of Spermidine synthase/SRM using anti-Spermidine synthase/SRM antibody (A01594-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermidine synthase/SRM antigen affinity purified polyclonal antibody (Catalog # A01594-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermidine synthase/SRM at approximately 34 kDa. The expected band size for Spermidine synthase/SRM is at 34 kDa.
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Western blot All lanes: SRM antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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