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SRP54 antibody

GTX115041
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSRP54
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Overview

  • Supplier
    GeneTex
  • Product Name
    SRP54 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6729
  • Target name
    SRP54
  • Target description
    signal recognition particle 54
  • Target synonyms
    SCN8, signal recognition particle subunit SRP54, signal recognition particle 54 kDa protein, signal recognition particle 54kD, signal recognition particle 54kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61011
  • Protein Name
    Signal recognition particle subunit SRP54
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Schürch C, Schaefer T, Müller JS, et al. SRP54 mutations induce congenital neutropenia via dominant-negative effects on XBP1 splicing. Blood. 2021,137(10):1340-1352. doi: 10.1182/blood.2020008115
    Read this paper

Datasheet

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Figure 1. Western blot analysis of SRP54 using anti-SRP54 antibody (A06189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP54 antigen affinity purified polyclonal antibody (Catalog # A06189-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRP54 at approximately 256 kDa. The expected band size for SRP54 is at 256 kDa.
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