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Srrt Polyclonal Antibody

CAC10671
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSRRT
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Overview

  • Supplier
    Biomatik
  • Product Name
    SRRT Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC; Recommended dilution: IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51593
  • Target name
    SRRT
  • Target description
    serrate, RNA effector molecule
  • Target synonyms
    ARS2; arsenate resistance protein 2; arsenate resistance protein ARS2; arsenite resistance protein; arsenite-resistance protein 2; ASR2; serrate; serrate RNA effector molecule homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXP5
  • Protein Name
    Serrate RNA effector molecule homolog
  • Scientific Description
    The Srrt Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARS2/SRRT antigen affinity purified polyclonal antibody (Catalog # A05444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARS2/SRRT at approximately 125 kDa. The expected band size for ARS2/SRRT is at 101 kDa.
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