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ProductsBack/SRRT Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA880129ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA880129ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA880129ESR1HU at dilution of 1:100

SRRT Antibody

CSB-PA880129ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSRRT
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRRT Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51593
  • Target name
    SRRT
  • Target description
    serrate, RNA effector molecule
  • Target synonyms
    ARS2; arsenate resistance protein 2; arsenate resistance protein ARS2; arsenite resistance protein; arsenite-resistance protein 2; ASR2; serrate; serrate RNA effector molecule homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXP5
  • Protein Name
    Serrate RNA effector molecule homolog
  • Scientific Description
    Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARS2/SRRT antigen affinity purified polyclonal antibody (Catalog # A05444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARS2/SRRT at approximately 125 kDa. The expected band size for ARS2/SRRT is at 101 kDa.
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