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Western Blot analysis of Jurkat cells using SRp20 Polyclonal Antibody
Western Blot analysis of Jurkat cells using SRp20 Polyclonal Antibody
Western Blot analysis of Jurkat cells using SRp20 Polyclonal Antibody

SRSF3 Antibody

CSB-PA004159
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSRSF3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRSF3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6428
  • Target name
    SRSF3
  • Target description
    serine and arginine rich splicing factor 3
  • Target synonyms
    SFRS3, SRp20, serine/arginine-rich splicing factor 3, epididymis secretory sperm binding protein, pre-mRNA splicing factor SRp20, pre-mRNA-splicing factor SRP20, splicing factor, arginine/serine-rich 3, splicing factor, arginine/serine-rich, 20-kD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP84103
  • Protein Name
    Serine/arginine-rich splicing factor 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SFRS3 using anti-SFRS3 antibody (PB9509). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRS3 antigen affinity purified polyclonal antibody (Catalog # PB9509) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRS3 at approximately 19 kDa. The expected band size for SFRS3 is at 19 kDa.
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