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ProductsBack/SRSF3 / SRP20 Antibody (80-160 aa, C-terminal)

SRSF3 / SRP20 Antibody (80-160 aa, C-terminal)

LS-C386182
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSRSF3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SRSF3 / SRP20 Antibody (80-160 aa, C-terminal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000), IF (1:200 - 1:1000), IHC (1:100 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6428
  • Target name
    SRSF3
  • Target description
    serine and arginine rich splicing factor 3
  • Target synonyms
    epididymis secretory sperm binding protein; pre-mRNA splicing factor SRp20; pre-mRNA-splicing factor SRP20; serine/arginine-rich splicing factor 3; SFRS3; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD; SRp20
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SFRS3 using anti-SFRS3 antibody (PB9509). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRS3 antigen affinity purified polyclonal antibody (Catalog # PB9509) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRS3 at approximately 19 kDa. The expected band size for SFRS3 is at 19 kDa.
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