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ProductsBack/SRY Antibody (clone OTI3C8)

SRY Antibody (clone OTI3C8)

LS-C337844
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSRY
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SRY Antibody (clone OTI3C8)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:100), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3C8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6736
  • Target name
    SRY
  • Target description
    sex determining region Y
  • Target synonyms
    essential protein for sex determination in human males; sex-determining region on Y; sex-determining region Y protein; SRXX1; SRXY1; TDF; TDY; testis-determining factor on Y; truncated sex-determining region Y protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRY using anti-SRY antibody (A00614-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRY antigen affinity purified polyclonal antibody (Catalog # A00614-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRY at approximately 26KD. The expected band size for SRY is at 24KD.
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