IP analysis of DDDDK tagged SSB overexpressed HEK293T lysate using GTX83558 SSB antibody [1E11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83558 SSB antibody [1E11]. Loading : 10 ug per lane Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_3600_WB_w_23061420_314.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83558 SSB antibody [1E11]. Loading : 10 ug per lane Dilution : 1:1000")
![WB analysis of various samples using GTX83558 SSB antibody [1E11]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_3659_WB_w_23061420_881.webp "WB analysis of various samples using GTX83558 SSB antibody [1E11]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with SSB plasmid (Right) or empty vector (Left) for 48 hrs using GTX83558 SSB antibody [1E11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_3733_WB_w_23061420_199.webp "WB analysis of HEK293T cells transfected with SSB plasmid (Right) or empty vector (Left) for 48 hrs using GTX83558 SSB antibody [1E11]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX83558 SSB antibody [1E11]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_3736_WB_w_23061420_483.webp "WB analysis of various cell lines using GTX83558 SSB antibody [1E11]. Loading : 35 ug per lane")
![FACS analysis of Jurkat cells using GTX83558 SSB antibody [1E11]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_71_FACS_w_23061420_111.webp "FACS analysis of Jurkat cells using GTX83558 SSB antibody [1E11]. Red : Primary antibody Blue : Negative control antibody")
![ICC/IF analysis of COS7 cells transiently transfected with SSB plasmid using GTX83558 SSB antibody [1E11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_716_ICCIF_w_23061420_493.webp "ICC/IF analysis of COS7 cells transiently transfected with SSB plasmid using GTX83558 SSB antibody [1E11].")
![ICC/IF analysis of HepG2 cells using GTX83558 SSB antibody [1E11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83558/GTX83558_717_ICCIF_w_23061420_409.webp "ICC/IF analysis of HepG2 cells using GTX83558 SSB antibody [1E11].")
IP analysis of DDDDK tagged SSB overexpressed HEK293T lysate using GTX83558 SSB antibody [1E11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
SSB antibody [1E11]
GTX83558
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetSSB
Overview
- SupplierGeneTex
- Product NameSSB antibody [1E11] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:50-1:100. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Concentration2.8 mg/ml
- ConjugateUnconjugated
- Gene ID6741
- Target nameSSB
- Target descriptionsmall RNA binding exonuclease protection factor La
- Target synonymsautoantigen La; La; la autoantigen; La ribonucleoprotein domain family, member 3; La/SSB; LARP3; lupus La antigen; lupus La protein; sjoegren syndrome type B antigen; Sjogren syndrome antigen B; SS-B; SS-B/La protein
- HostMouse
- IsotypeIgG2a
- Protein IDP05455
- Protein NameLupus La protein
- Scientific DescriptionBinds to the 3 poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
- ReactivityCanine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203