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ProductsBack/SSB Mouse Monoclonal Antibody [Clone ID: OTI1E11]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSB (Cat# RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB(Cat# TA500406). Positive lysates [LY401091] (100ug) and [LC401091] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSB (Cat# RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB(Cat# TA500406). Positive lysates [LY401091] (100ug) and [LC401091] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSB (Cat# RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB(Cat# TA500406). Positive lysates [LY401091] (100ug) and [LC401091] (20ug) can be purchased separately from OriGene.

SSB Mouse Monoclonal Antibody [Clone ID: OTI1E11]

CF500406
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetSSB
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SSB Mouse Monoclonal Antibody [Clone ID: OTI1E11]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E11
  • Gene ID6741
  • Target name
    SSB
  • Target description
    small RNA binding exonuclease protection factor La
  • Target synonyms
    LARP3, La, La/SSB, lupus La protein, La ribonucleoprotein domain family, member 3, SS-B, SS-B/La protein, Sjogren syndrome antigen B, autoantigen La, la autoantigen, lupus La antigen, sjoegren syndrome type B antigen
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP05455
  • Protein Name
    Lupus La protein
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SSB using anti-SSB antibody (A00705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSB antigen affinity purified polyclonal antibody (Catalog # A00705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSB at approximately 47 kDa. The expected band size for SSB is at 47 kDa.
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