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ProductsBack/SSB Mouse Monoclonal Antibody [Clone ID: OTI2C8]
Simple Western™ analysis of endogenous protein SSB from HELA lysates (0.2 mg/mL) using SSB Mouse Monoclonal Antibody #TA500426. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein SSB from HELA lysates (0.2 mg/mL) using SSB Mouse Monoclonal Antibody #TA500426. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein SSB from HELA lysates (0.2 mg/mL) using SSB Mouse Monoclonal Antibody #TA500426. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

SSB Mouse Monoclonal Antibody [Clone ID: OTI2C8]

CF500426
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetSSB
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SSB Mouse Monoclonal Antibody [Clone ID: OTI2C8]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2C8
  • Gene ID6741
  • Target name
    SSB
  • Target description
    small RNA binding exonuclease protection factor La
  • Target synonyms
    LARP3, La, La/SSB, lupus La protein, La ribonucleoprotein domain family, member 3, SS-B, SS-B/La protein, Sjogren syndrome antigen B, autoantigen La, la autoantigen, lupus La antigen, sjoegren syndrome type B antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05455
  • Protein Name
    Lupus La protein
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SSB mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)
  • Reactivity
    Canine, Human, Monkey
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SSB using anti-SSB antibody (A00705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSB antigen affinity purified polyclonal antibody (Catalog # A00705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSB at approximately 47 kDa. The expected band size for SSB is at 47 kDa.
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