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Western Blot analysis of HeLa cells using SSBP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using SSBP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using SSBP1 Polyclonal Antibody

SSBP1 Antibody

CSB-PA004162
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSSBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SSBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6742
  • Target name
    SSBP1
  • Target description
    single stranded DNA binding protein 1
  • Target synonyms
    Mt-SSB, OPA13, SOSS-B1, SSBP, mtSSB, single-stranded DNA-binding protein, mitochondrial, PWP1-interacting protein 17, single-stranded DNA binding protein 1, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04837
  • Protein Name
    Single-stranded DNA-binding protein, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SSBP1 using anti-SSBP1 ntibody (A05166-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSBP1 antigen affinity purified polyclonal antibody (Catalog # A05166-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSBP1 at approximately 16 kDa. The expected band size for SSBP1 is at 17-18 kDa.
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