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IP analysis of MCF-7 cell lysate using GTX55806 SSBP1 antibody. Antibody amount : 1microg / 200microg lysate Dilution : 1:1000
IP analysis of MCF-7 cell lysate using GTX55806 SSBP1 antibody. Antibody amount : 1microg / 200microg lysate Dilution : 1:1000
IP analysis of MCF-7 cell lysate using GTX55806 SSBP1 antibody. Antibody amount : 1microg / 200microg lysate Dilution : 1:1000

SSBP1 antibody

GTX55806
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSSBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SSBP1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6742
  • Target name
    SSBP1
  • Target description
    single stranded DNA binding protein 1
  • Target synonyms
    mtSSB; Mt-SSB; OPA13; PWP1-interacting protein 17; single-stranded DNA binding protein 1, mitochondrial; single-stranded DNA-binding protein, mitochondrial; SOSS-B1; SSBP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04837
  • Protein Name
    Single-stranded DNA-binding protein, mitochondrial
  • Scientific Description
    SSBP1 is a housekeeping gene involved in mitochondrial biogenesis (Tiranti et al., 1995 [PubMed 7789991]). It is also a subunit of a single-stranded DNA (ssDNA)-binding complex involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. Wu WY et al., 2022 Jun, Redox Biol
    Read more

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Figure 1. Western blot analysis of SSBP1 using anti-SSBP1 ntibody (A05166-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSBP1 antigen affinity purified polyclonal antibody (Catalog # A05166-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSBP1 at approximately 16 kDa. The expected band size for SSBP1 is at 17-18 kDa.
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