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Western blot All lanes: Alpha-2, 8-sialyltransferase 8B antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Western blot All lanes: Alpha-2, 8-sialyltransferase 8B antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Western blot All lanes: Alpha-2, 8-sialyltransferase 8B antibody at 8ug/ml Lane 1: Mouse heart tissue Lane 2: HepG2 whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa

ST8SIA2 Antibody

CSB-PA852840ESR2HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetST8SIA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ST8SIA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8128
  • Target name
    ST8SIA2
  • Target description
    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
  • Target synonyms
    HsT19690, SIAT8-B, SIAT8B, ST8SIA-II, ST8SiaII, STX, alpha-2,8-sialyltransferase 8B, ST8 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase 2, alpha-2,8-sialyltransferase 8B 1, sialyltransferase 8 (alpha-2, 8-sialytransferase) B, sialyltransferase 8B, sialyltransferase St8Sia II, sialyltransferase X
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92186
  • Protein Name
    Alpha-2,8-sialyltransferase 8B
  • Scientific Description
    May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ST8SIA2 using anti-ST8SIA2 antibody (A05894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST8SIA2 antigen affinity purified polyclonal antibody (Catalog # A05894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST8SIA2 at approximately 39 kDa. The expected band size for ST8SIA2 is at 42 kDa.
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