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Anti-ST8SIA2 Antibody

CAB7748
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetST8SIA2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-ST8SIA2 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID8128
  • Target name
    ST8SIA2
  • Target description
    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
  • Target synonyms
    HsT19690, SIAT8-B, SIAT8B, ST8SIA-II, ST8SiaII, STX, alpha-2,8-sialyltransferase 8B, ST8 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase 2, alpha-2,8-sialyltransferase 8B 1, sialyltransferase 8 (alpha-2, 8-sialytransferase) B, sialyltransferase 8B, sialyltransferase St8Sia II, sialyltransferase X
  • Host
    Rabbit
  • Protein IDQ92186
  • Protein Name
    Alpha-2,8-sialyltransferase 8B
  • Scientific Description
    ST8SIA2 Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ST8SIA2 using anti-ST8SIA2 antibody (A05894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST8SIA2 antigen affinity purified polyclonal antibody (Catalog # A05894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST8SIA2 at approximately 39 kDa. The expected band size for ST8SIA2 is at 42 kDa.
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