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WB analysis of human breast lysate using GTX88199 STAP2 (aa391-403) antibody, C-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human breast lysate using GTX88199 STAP2 (aa391-403) antibody, C-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human breast lysate using GTX88199 STAP2 (aa391-403) antibody, C-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer

STAP2 (aa391-403) antibody, C-term

GTX88199
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSTAP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    STAP2 (aa391-403) antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.3-1microg/ml. IHC-P: 3.75microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55620
  • Target name
    STAP2
  • Target description
    signal transducing adaptor family member 2
  • Target synonyms
    BKS; breast tumor kinase substrate; brk kinase substrate; BRK substrate; signal-transducing adaptor protein 2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UGK3
  • Protein Name
    Signal-transducing adaptor protein 2
  • Scientific Description
    This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAP2 using anti-STAP2 antibody (A08413-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAP2 antigen affinity purified polyclonal antibody (Catalog # A08413-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAP2 at approximately 50-55 kDa. The expected band size for STAP2 is at 45 kDa.
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